The quality of buccal mucosal swab preservation and its impact on DNA sequencing tests
Buccal swab samples tend to have fewer contaminants than other DNA samples, such as blood or saliva
Alexandre Campos Banari – Scientist | Naila Cristina Soler Camargo – Scientist (Scientific Collaboration)
Mouth swab collection is a non-invasive method for obtaining biological material for DNA/RNA sequencing research. This makes tissue extraction a more comfortable and less painful procedure for the patient. In this context, KolplaGene, Kolplast’s solution for preserving genetic material, stands out for its high performance in obtaining samples with a high concentration of DNA from buccal mucosa swabs.
Mouth swab samples, when preserved in KolplaGene, can be easily stored for up to 30 days at room temperature, helping to overcome logistical obstacles related to distance and time between sample collection and processing. Another advantage is that buccal swab samples tend to have fewer contaminants than other DNA samples, such as blood or saliva. This can reduce the cost and time needed to extract and purify the DNA and conduct its subsequent sequencing.
The data generated by the sequencer can be used to identify polymorphisms associated with diseases and to understand the complex interactions between microorganisms in different environments. By analyzing an individual’s DNA sequence, it is possible to determine whether there is a known polymorphism that may be the cause or aggravating factor of a clinical condition, allowing for the early diagnosis of hereditary diseases and the selection of more appropriate therapies. Or to determine the importance and function of microorganisms present in biological samples, such as soil, water and human body tissues, in order to maintain the health and balance of the ecosystem.
The use of high-throughput sequencing technologies in diagnostic medicine and microbiome analysis has enabled a more comprehensive understanding of diseases and the development of new strategies for personalized prevention and treatment, as well as opening up new research opportunities in areas such as genomics, molecular biology and microbiology.